PCNA Monoclonal Antibody
Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Catalogue Numbers: MB66872-50, MB66872-100
Sizes: 50µl, 100µl
Swiss-Prot: P12004
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand .Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways .Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification and Purity: This antibody is purified through a protein G column.
Specificity: Recognizes endogenous levels of PCNA protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PCNA expression in C2C12 (A), C6 (B), Hela (C), L929 (D), MCF7 (E) whole cell lysates., Immunohistochemical analysis of PCNA staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Bioworld Molecular Weight: ~ 36 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Proliferating cell nuclear antigen; PCNA; Cyclin
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PCNA. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified