PEF1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11392-50, MB11392-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: Q9UBV8
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB,IP
All Applications: WB: 1/500-1/1000 IP: 1/20
Background: Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 .
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PEF1 in K562, rat Brain, C6, 3T3, HelaLysates using PEF1 antibody.
Bioworld Molecular Weight: Calculated MW: 30 kDa; Observed MW: 30 kDa
Specificity: IgG
Alternative Name: penta-EF-hand domain containing 1; ABP32; PEF1A
Immunogen: A synthetic peptide of human PEF1
Conjugate: Unconjugated
Modification: Unmodified