Perforin 1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB66237-50, MB66237-100
Product: Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Swiss-Prot: P14222
Host: Mouse
Reactivity: Human
Applications: IHC
All Applications: IHC (1/100 - 1/300)
Background: Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells . Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).
Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells . Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemical analysis of Perforin 1 staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Bioworld Molecular Weight: ~ 70-75 kDa
Specificity: Recognizes endogenous levels of Perforin 1 protein.
Alternative Name: PFP; Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; PFP
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Perforin 1. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified