Phospho-CBL (Tyr774) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10426-50, MB10426-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P22681
Host: Rabbit
Reactivity: Human, Rat
Applications: WB,IP
All Applications: WB: 1/500-1/1000 IP: 1/20
Background: This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-CBL in K562, C6, HelaLysates using Phospho-CBL antibody.
Bioworld Molecular Weight: Calculated MW: 100 kDa; Observed MW: 120 kDa
Specificity: IgG
Alternative Name: CBL; CBL2; RNF55; E3 ubiquitin-protein ligase CBL; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Tyr774 of human CBL
Conjugate: Unconjugated
Modification: Phosphorylated