Phospho-eIF4E (Ser209) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10868-50, MB10868-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P06730
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-F, IHC-P, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 25 kDa; Observed MW: 25 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of eIF4E in K562, rat Brain, C6, 3T3 lysates using Phospho-eIF4E antibody., Immunocytochemistry analysis of eIF4E in Hela using eIF4E antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-eIF4E antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E
Conjugate: Unconjugated
Modification: Phosphorylated