Phospho-Histone H3 (Ser10/Thr11) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11866-50, MB11866-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P68431
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-F, IHC-P, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Background: H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunocytochemistry analysis of Phospho-Histone H3 in Hela using Phospho-Histone H3 antibody, and DAPI., Western blot analysis of Phospho-Histone H3 in 3T3 lysates using Phospho-Histone H3 antibody., Western blot analysis of Histone H3 in Hela, A549, HL-60, C6 lysates using Histone H3 antibody, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: H3 histone; family 3A; H3 histone; family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser10/Thr11 of human Histone H3
Conjugate: Unconjugated
Modification: Phosphorylated