Phospho-MCM2 (Ser41) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10422-50, MB10422-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P49736
Host: Rabbit
Reactivity: Human, Rat, Hamster
Applications: WB, IHC-P, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 102 kDa; Observed MW: 125 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-MCM2 in Jurkat, C6, CHO-K1, Hela lysates using Phospho-MCM2 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using MCM2 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.
Alternative Name: MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser41 of human MCM2
Conjugate: Unconjugated
Modification: Phosphorylated