Phospho-Nucleophosmin (Thr199) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11879-50, MB11879-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P06748
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, IHC-P, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Background: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 33 kDa; Observed MW: 38 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Nucleophosmin in C6, Ramos lysates using Phospho-Nucleophosmin antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Nucleophosmin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: B23; NPM
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr199 of human Nucleophosmin
Conjugate: Unconjugated
Modification: Phosphorylated