Phospho-p27 Kip 1 (Ser10) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11350-50, MB11350-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P46527
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Background: The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 27 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-p27 KIP 1 in Hela, 3T3 lysates using Phospho-p27 Kip 1 antibody., Western blot analysis of Phospho-p27 KIP 1 in C6 lysates using Phospho-p27 KIP 1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using p27 KIP 1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Conjugate: Unconjugated
Modification: Phosphorylated