PP1A / PPP1CA (N-terminus) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB0084-50, MB0084-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P62136
Host: Mouse
Reactivity: Human
Applications: WB, IHC ICC
All Applications: WB: 1:1000
ICC: 1:200~400
IHC: 1:500
Background: Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role. in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of PP1A (N-terminus) and does not cross-react with related proteins
Bioworld Molecular Weight: Predicted band size: 38KDa
Observed band size: 38KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot detection of PPP1CA in MCF7, K562, Hela, HEPG2 and Jurkat cell lysates using PPP1CA antibody (1:1000 diluted)., IHC of paraffin-embedded human breast cancer using anti- PPP1CA diluted 1/500-1/1000., Immunocytochemistry stain of Hela using PPP1CA antibody (1:300).
Alternative Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A; PPP1CA; PPP1A
Immunogen: Synthetic peptide, corresponding to the N-terminus of Human PP1A / PPP1CA.
Conjugate: Unconjugated
Modification: Unmodified