PRAS40 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB12204-50, MB12204-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: Q96B36
Host: Rabbit
Reactivity: Human, Rat
Applications: WB,IP
All Applications: WB: 1/500-1/1000 IP: 1/20
Background: PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PRAS40 in Hela, A549, C6Lysates using PRAS40 antibody.
Bioworld Molecular Weight: Calculated MW: 27 kDa; Observed MW: 40 kDa
Specificity: IgG
Alternative Name: AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
Immunogen: A synthetic peptide of human PRAS40
Conjugate: Unconjugated
Modification: Unmodified