Rab9 Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9854M-50, BS9854M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P51151
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF
All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
Background: The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of Rab9 and does not cross-react with related proteins.
Bioworld Molecular Weight: ~ 23 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Rab9 Rabbit mAb at 1:1000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: K562 whole cell lysate, ICC staining Rab9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
Alternative Name: Ras-related protein Rab-9A; RAB9A; RAB9
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Unmodified