Rad21 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB12218-50, MB12218-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: O60216
Host: Rabbit
Reactivity: Human, Hamster
Applications: WB, IHC-F, IHC-P, ICC/IF
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Background: Rad21 is one of the major cohesin subunits that holds sister chromatids together until anaphase, when proteolytic cleavage by separase, a caspaselike enzyme, allows chromosomal separation. Rad21 interacts with Rec8 to form a cohesin complex that functions in sister chromatid alignment. Rad21 is also involved in the repair of double-strand breaks in DNA and is essential for mitotic growth. Rad21 undergoes a C-terminal cleavage induced by diverse stimuli right before apoptosis.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 72 kDa; Observed MW: 130 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunocytochemistry analysis of Rad21 in Hela using Rad21 antibody, and DAPI., Western blot analysis of Rad21 in Hela, CHO-K1, Ramos lysates using Rad21 antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Rad21 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: RAD21; HR21; KIAA0078; NXP1; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog
Immunogen: A synthetic peptide of human Rad21
Conjugate: Unconjugated
Modification: Unmodified