RAD51A Monoclonal Antibody
Catalogue Numbers: MB67078-50, MB67078-100
Sizes: 50µl, 100µl
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: Q06609
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human disease, including cancer (reviewed in 1).
DSBs that arise during S or G2 phase are repaired via homologous recombination (HR), using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homologue of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (reviewed in 2,3). BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D) and they all appear to be required for efficient HR.
Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk. Some studies have implicated Rad51 as a potential marker for pancreatic cancer.
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of RAD51A protein.
Extra Notes: Western blot analysis of RAD51A expression in Jurkat (A), Hela (B), NIH3T3 (C), C6 (D), PC12 (E) whole cell lysates.
Bioworld Molecular Weight: ~ 37 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
Immunogen: Recombinant fusion protein of human RAD51A. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified