RAD9A Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB0162-50, MB0162-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q99638
Host: Mouse
Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IP,
All Applications: WB: 1:500~1000
IP: 1:50~100
Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3´-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3´->5´ double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of RAD9A and does not cross-react with related proteins
Bioworld Molecular Weight: Predicted band size: 43KDa
Observed band size: 55KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot detection of RAD9A in Hela, MCF7, 3T3, COS7 and Rat testis cell lysates using RAD9A antibody (1:500 diluted)., Immunoprecipitation analysis of Hela and 3T3 cell lysates using RAD9A antibody
Alternative Name: Cell cycle checkpoint control protein; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9
Immunogen: Recombinant full length Human RAD9A.
Conjugate: Unconjugated
Modification: Unmodified