Raf1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11505-50, MB11505-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P04049
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF
All Applications: WB: 1/500-1/1000 IF: 1/50-1/200
Background: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 73 kDa; Observed MW: 73 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Raf1 in C6, 3T3, Hela lysates using Raf1 antibody., Immunocytochemistry analysis of Raf1 in MCF-7 using Raf1 antibody, and DAPI
Alternative Name: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Immunogen: A synthetic peptide of human Raf1
Conjugate: Unconjugated
Modification: Unmodified