Raf1 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A19638-20, A19638-100
Citations, Manuals and SDS Available upon request.
Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Synonyms: NS5; CRAF; Raf-1; c-Raf; CMD1NN; Raf1
GeneID: 5894
Swiss-Prot: P04049
Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB, IP
Calculated Molecular Weight: 73kDa
Observed Molecular Weight: 73kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Raf1 (P04049).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Research Areas: Protein phosphorylation, Cancer, Signal Transduction, G protein signaling, G-Protein-Coupled Receptors Signaling to MAPK Erk, Kinase, Serine threonine kinases, ErbB-HER Signaling Pathway, MAPK-Erk Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Inhibition of Apoptosis, Growth factors, ESC Pluripotency and Differentiation, Endocrine Metabolism, Mitochondrial metabolism, Mitochondrial markers, Insulin Receptor Signaling Pathway, Immunology Inflammation, B Cell Receptor Signaling Pathway, T Cell Receptor Signaling Pathway, Jak-Stat-IL-6 Receptor Signaling Pathway, Neuroscience
Recommended Dilutions: WB: 1:500 - 1:2000；IP: 1:500 - 1:0000
Modifications: Unmodified
Conjugate: Unconjugated
Cellular Location: Cell Membrane, Cytoplasm, Mitochondrion, Nucleus
Positive Samples: MCF7, Jurkat, HepG2, HeLa, NIH/3T3, Rat liver
Keywords: 5894, sc-227, sc-133, sc-7198, c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1, CMD1NN, Raf-1, c-Raf, Raf-1 proto-oncogene, serine/threonine kinase, RAF proto-oncogene serine/threonine-protein kinase, C-Raf proto-oncogene, Oncogene RAF1, proto-oncogene c-RAF, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, P04049, Raf-1 Proto-Oncogene, Serine/Threonine Kinase, V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1, C-Raf Proto-Oncogene, Proto-Oncogene C-RAF, V-Raf-1 Murine Leukemia Viral Oncogene-Like Protein 1, RAF Proto-Oncogene Serine/Threonine-Protein Kinase, Raf Proto-Oncogene Serine/Threonine Protein Kinase, EC 2.7.11.1, C-Raf, CRaf, 免疫, 炎症, 天然免疫, 感染, 病毒, 自身免疫, CLR, Lectin, Raf1
Symbol: RAF1
Category: Monoclonal Antibodies
Research Use Only