RNA Polymerases I/II/III Subunit ABC1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11439-50, MB11439-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P19388
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100
Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process .
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 25 kDa; Observed MW: 25 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of POLR2E in K562, C6, 3T3, Hela lysates using RNA Polymerases I/II/III Subunit ABC1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using POLR2E antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.
Alternative Name: RPB5; XAP4; RPABC1; hRPB25; hsRPB5
Immunogen: A synthetic peptide of human POLR2E
Conjugate: Unconjugated
Modification: Unmodified