RNF20 Monoclonal Antibody
Catalogue Numbers: MB67128-50, MB67128-100
Sizes: 50µl, 100µl
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: Q5VTR2
Host: Mouse
Reactivity: Human
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1. Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin. Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified . These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Researchers have shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways. Indeed, investigators have shown that loss of RNF20-RNF40 function promotes replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion .
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of RNF20 protein.
Extra Notes: Western blot analysis of RNF20 expression in Hela (A), Jurkat (B), HL60 (C), U87MG (D) whole cell lysates.
Bioworld Molecular Weight: ~ 120 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: BRE1A; E3 ubiquitin-protein ligase BRE1A; BRE1-A; hBRE1; RING finger protein 20
Immunogen: Recombinant fusion protein of human RNF20. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified