RNF4 Monoclonal Antibody
Catalogue Numbers: MB67059-50, MB67059-100
Sizes: 50µl, 100µl
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: P78317
Host: Mouse
Reactivity: Human
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation.
Regulates the degradation of several proteins including PML and the transcriptional activator PEA3.
Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation.
Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1.
Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.
Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97.
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of RNF4 protein.
Extra Notes: Western blot analysis of RNF4 expression in LNCap (A), PC3 (B), human testis tissue (C) whole cell lysates.
Bioworld Molecular Weight: ~ 35 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: SNURF; E3 ubiquitin-protein ligase RNF4; RING finger protein 4; Small nuclear ring finger protein; Protein SNURF
Immunogen: Recombinant fusion protein of human RNF4. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified