RPA70/RPA1 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A3367-20, A3367-100
Citations, Manuals and SDS Available upon request.
Background: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
Synonyms: HSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6; RPA70/RPA1
GeneID: 6117
Swiss-Prot: P27694
Reactivity: Human, Rat
Calculated Molecular Weight: 68kDa
Observed Molecular Weight: 70kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IP, ChIP
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:2000 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells | ChIP: 5μg antibody for 10μg-15μg of Chromatin
Research Areas: Epigenetics Nuclear Signaling, Transcription Factors, DNA Damage Repair
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: HeLa, U-251MG, Rat testis
Cellular Location: Nucleus, PML Body
Category: Monoclonal Antibodies
Citations, Manuals and SDS Available upon request.