RPE65 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A9615-20, A9615-100
Citations, Manuals and SDS Available upon request.
Background: The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital.
Synonyms: p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65; RPE65
GeneID: 6121
Swiss-Prot: Q16518
Reactivity: Mouse, Rat
Calculated Molecular Weight: 61kDa
Observed Molecular Weight: 70kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human RPE65 (Q16518).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Tested Applications: ELISA, WB, IHC-P, IF/ICC
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. | Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200
Research Areas: Cancer, Signal Transduction, Endocrine Metabolism, Neuroscience, Cell Type Marker
Modifications: Unmodified
Conjugate: Unconjugated
Positive Samples: Rat eye
Cellular Location: Cell Membrane, Cytoplasm, Lipid-anchor, Microsome Membrane
Category: Monoclonal Antibodies
Citations, Manuals and SDS Available upon request.