USP7 (C-terminus) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB0073-50, MB0073-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q93009
Host: Mouse
Reactivity: Human
Applications: WB, ICC
All Applications: WB: 1:1000
ICC: 1:100~200
Background: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes. Herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) is an important deubiquitinase belonging to USP subfamily. A key HAUSP function is to bind and deubiquitinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins. In addition to regulating essential components of the p53 pathway, HAUSP also modifies other ubiquitinylated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR.
Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of USP7 (C-terminus) and does not cross-react with related proteins
Bioworld Molecular Weight: Predicted band size: 129KDa
Observed band size: 140KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot detection of USP7 (C-terminus) antibody in Hela cell lysates using USP7 (C-terminus) antibody (1:1000 diluted)., Immunocytochemistry of HeLa cells using anti- USP7 (C-terminus) antibody diluted 1:150.
Alternative Name: TEF1; HAUSP; Ubiquitin carboxyl-terminal hydrolase 7; Deubiquitinating enzyme 7; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7
Immunogen: Synthetic peptide, corresponding to the C-terminus of Human USP7o.
Conjugate: Unconjugated
Modification: Unmodified