Multiplex Assay Kit for Hepcidin (Hepc), etc. by FLIA (Flow Luminescence Immunoassay)
Size: 96T
Catalogue Number: LMB979Po-96
Citations, Manuals and MSDS Available upon request.
Detection range: 58.59-60000pg/mL
Application: FLIA Kit for Antigen Detection.
Organism species: Sus scrofa; Porcine (Pig)
Sensitivity: The minimum detectable dose of this kit is typically less than 19.53 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3h
Method: Competitive Inhibition
Specificity: This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc), etc. by FLIA (Flow Luminescence Immunoassay).No significant cross-reactivity or interference between Hepcidin (Hepc), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Assay procedure summary: 1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads, and 50μL Detection Reagent A, incubate 90min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle: Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverseproportional to the concentration of analytes of interest in the sample.
Alternative Names: HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
Item Name: Hepcidin
Research Field: Metabolic pathway; Endocrinology; Hematology; Genetic science;
Uniprot: Q8MJ80
Research Use Only