N6-methyladenosine / m6A Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A17924-20, A17924-100
Citations, Manuals and MSDS Available upon request.
Background: Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development. (PMID: 30416848; PMID: 24662220; PMID: 30429466)
Category: Other Modified Antibodies
Applications: DB, IF/ICC, ELISA, meRIP, Nucleotide Array
Cross-reactivity: Species independent
Immunogen: Chemical compounds corresponding to N6-methyladenosine / m6A.
Alternate Names: N6-methyladenosine; m6A; N6-methyladenosine / m6A
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: DB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. meRIP, 1:50 - 1:200
NCBI Alias: m6A
Research Use Only