Phospho-Chk2-T68 Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: AP1407-20, AP1407-100
Citations, Manuals and SDS Available upon request.
Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Synonyms: CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; Phospho-Chk2-T68
GeneID: 11200
Swiss Prot: O96017
Tested Applications: ELISA, IHC-P
Reactivity: Human, Mouse, Rat
Calculated Molecular Weight: 61kDa
Observed Molecular Weight: Refer to figures
Immunogen: A synthetic phosphorylated peptide around T68 of human Chk2 (NP_009125.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: IHC-P: 1:500 - 1:1000
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair, Protein phosphorylation, Cancer, Signal Transduction, Kinase, Serine threonine kinases, ATM Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Cell Cycle, Cell Cycle Control-G1 S Checkpoint, Cell Cycle Control-G2 M DNA Damage Checkpoint
Modifications: Phospho T68
Conjugate: Unconjugated
Cellular Location: Nucleus, PML Body, Nucleoplasm
Keywords: 11200, sc-8813, sc-8812, sc-9064, CDS1, CHEK2, Chk2, HuCds1, LFS2, PP1425, RAD53, CHK2, hCds1, checkpoint kinase 2, serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, cds1 homolog, checkpoint-like protein CHK2, O96017, Checkpoint Kinase 2, CHK2 Checkpoint Homolog, Cds1 Homolog, HCds1, Serine/Threonine-Protein Kinase Chk2, CHK2 (Checkpoint, S.Pombe) Homolog, CHK2 Checkpoint Homolog (S. Pombe), Checkpoint-Like Protein CHK2, EC 2.7.11.1, Hucds1, Phospho-CHEK2-S19, 磷酸化, Phospho-Chk2-T68
Symbol: CHEK2
Category: Phosphorylated Antibodies
Research Use Only