Phospho-RPA2-S4/S8 Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: AP1102-20, AP1102-100
Citations, Manuals and SDS Available upon request.
Background: This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.
Synonyms: REPA2; RPA32; RP-A p32; RP-A p34; Phospho-RPA2-S4/S8
GeneID: 6118
Swiss Prot: P15927
Tested Applications: ELISA, WB
Reactivity: Rat
Calculated Molecular Weight: 29kDa
Observed Molecular Weight: 36kDa
Immunogen: A synthetic phosphorylated peptide around S4 & S8 of human RPA2 (NP_002937.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair
Modifications: Phospho S4/S8
Positive Samples: C6
Cellular Location: Nucleus, PML Body,
Keywords: 6118, sc-46501, sc-46626, sc-14691, sc-14692, sc-28709, RPA2, REPA2, RP-A p32, RP-A p34, RPA32, replication protein A2, replication protein A 32 kDa subunit, RF-A protein 2, replication factor A protein 2, replication protein A 34 kDa subunit, 32kDa, P15927, Replication Protein A2, Replication Protein A 34 KDa Subunit, Replication Factor A Protein 2, RF-A Protein 2, RP-A P32, RP-A P34, Replication Protein A 32 KDa Subunit, Replication Protein A2 (32kD), RPA34, RP-Ap32, RP-Ap34, 磷酸化, Phospho-RPA2-S4/S8
Symbol: RPA2
Category: Phosphorylated Antibodies
Research Use Only