Phospho-Smad2-S465/S467 Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: AP0925-20, AP0925-100
Citations, Manuals and SDS Available upon request.
Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Synonyms: JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; Phospho-Smad2-S465/S467
GeneID: 4087
Swiss Prot: Q15796
Tested Applications: ELISA, WB
Reactivity: Human, Mouse
Calculated Molecular Weight: 52kDa
Observed Molecular Weight: 60kDa
Immunogen: A synthetic phosphorylated peptide around S465 & S467 of human Smad2 (NP_005892.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:2000
Research Areas: Epigenetics Nuclear Signaling, Transcription Factors, Protein phosphorylation, Cancer, Signal Transduction, Cell Biology Developmental Biology, Growth factors, TGF-b-Smad Signaling Pathway, ESC Pluripotency and Differentiation, Stem Cells, Cardiovascular, Angiogenesis
Modifications: Phospho S465/S467
Positive Samples: HeLa
Cellular Location: Cytoplasm, Nucleus
Keywords: 4087, sc-6200, sc-6033, sc-6032, sc-6202, hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2, JV18-1, hMAD-2, mothers against decapentaplegic homolog 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2, Q15796, SMAD Family Member 2, MAD Homolog 2, HMAD-2, HSMAD2, MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila), Mothers Against DPP Homolog 2 (Drosophila), Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, Sma- And Mad-Related Protein 2, Mother Against DPP Homolog 2, Mad-Related Protein 2, Smad2, 磷酸化, Phospho-Smad2-S465/S467
Symbol: SMAD2
Category: Phosphorylated Antibodies
Research Use Only