ACLY Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB66502-50, MB66502-100
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Swiss-Prot: P53396
Host: Mouse
Reactivity: Human, Mouse, Monkey
Applications: WB, IF/ICC, FC
All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), FC (1/50 - 1/100)
Background: ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis . Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase . It is phosphorylated by GSK-3 on Thr446 and Ser450 . Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt . Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme .
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of ACLY protein.
Bioworld Molecular Weight: ~ 125 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ACLY expression in NIH3T3 (A), K562 (B), COS7 (C), Hela (D) whole cell lysates., Immunofluorescent analysis of ACLY staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Alternative Name: ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme
Immunogen: Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
Conjugate: Unconjugated
Modification: Unmodified