ACOT12 Polyclonal Antibody
Catalogue Numbers:BS65223-50, BS65223-100
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Swiss-Prot: Q8WYK0
Host: Rabbit
Reactivity: Human
Applications: IHC-p, IF/ICC, ELISA
All Applications: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Background: catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate., function: Hydrolyzes acetyl-CoA to acetate and CoA., pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ACOT12 Polyclonal Antibody detects endogenous levels of ACOT12 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide., Immunofluorescence analysis of A549 cells, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide., Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
Alternative Name: ACOT12; CACH; CACH1; STARD15; Acyl-coenzyme A thioesterase 12; Acyl-CoA thioesterase 12; Acyl-CoA thioester hydrolase 12; Cytoplasmic acetyl-CoA hydrolase 1; CACH-1; hCACH-1; START domain-containing protein 15; StARD15
Immunogen: The antiserum was produced against synthesized peptide derived from human ACOT12. AA range:281-330
Conjugate: Unconjugated
Modification: Unmodified