ACOT12 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS67472-50, BS67472-100
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: Q8WYK0
Host: Rabbit
Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC, IF/ICC
All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)
Background: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels .Preferentially hydrolyzes acetyl-CoA .
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of ACOT12 protein.
Bioworld Molecular Weight: ~ 62 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ACOT12 expression in AML12 (A), rat liver (B), HepG2 (C), HEK293T (D) whole cell lysates., Immunohistochemical analysis of ACOT12 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of ACOT12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Alternative Name: CACH; CACH1; STARD15; Acyl-coenzyme A thioesterase 12; Acyl-CoA thioesterase 12; Acyl-CoA thioester hydrolase 12; Cytoplasmic acetyl-CoA hydrolase 1; CACH-1; hCACH-1; START domain-containing protein 15; StARD15
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACOT12. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified