ACOX1 Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB66309-50, MB66309-100
Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Swiss-Prot: Q15067
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC, IP,
All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)
Background: The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of ACOX1 protein.
Bioworld Molecular Weight: ~ 50, 72 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ACOX1 expression in Raw264.7 (A), mouse liver (B) whole cell lysates., Immunofluorescent analysis of ACOX1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Alternative Name: ACOX; Peroxisomal acyl-coenzyme A oxidase 1; AOX; Palmitoyl-CoA oxidase; Straight-chain acyl-CoA oxidase; SCOX
Immunogen: Recombinant protein of mouse ACOX1
Conjugate: Unconjugated
Modification: Unmodified