Actin-κ (T121) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9201-50, BS9201-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9BYX7
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Actin-κ (T121) polyclonal antibody detects endogenous levels of Actin-κ protein.
Bioworld Molecular Weight: ~ 42 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry (IHC) analyzes of Actin-κ (T121) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Actin-κ (T121) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50, showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; FKSG30; ACTBL3; POTEKP
Immunogen: Synthetic peptide, corresponding to amino acids 90-134 of Human Actin-κ.
Conjugate: Unconjugated
Modification: Unmodified