Actinin α Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90023-50, BS90023-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P12814(Human) Q7TPR4(Mouse) Q9Z1P2(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:100
IHC:1:50-1:200
FC:1:50-1:100
Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: Actinin α polyclonal antibody detects endogenous levels of Actinin α protein.
Bioworld Molecular Weight: 103 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Fig1: Western blot analysis of alpha Actinin on different lysates using anti-alpha Actinin antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Rat colon tissue
Lane 3: Mouse colon tissue, ICC staining alpha Actinin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: ACTN1, actinin 1 smooth muscle, Actinin alpha 1, actinin, alpha 1, ACTN 1, Actn1, ACTN1, Alpha Actinin 1, Alpha actinin cytoskeletal isoform, Alpha-actinin cytoskeletal isoform, Alpha-actinin-1, BDPLT15, F actin cross linking protein, F-actin cross-linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1, Non-muscle alpha-actinin-1,
Immunogen: Recombinant protein within human alpha Actinin aa 350-650.
Conjugate: Unconjugated
Modification: Unmodified