Actinin-α1/2/3/4 (W48) Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS2206-50, BS2206-100
Sizes: 50µl, 100µl
Swiss-Prot: P12814/P35609/Q08043/O43707
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Actinin-α1/2/3/4 (W48) polyclonal antibody detects endogenous levels of total α-actinin protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Actinin-α1/2/3/4 (W48) pAb at 1:500 dilution
Lane 1: The Kidney tissue lysate of Mouse (40ug)
Lane 2: The Brain tissue lysate of Rat (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: PC3 whole cell lysate (40ug)
Lane 5: U-87MG whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Actinin-α1/2/3/4 (W48) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 103 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: pan-α-Actinin; α-Actinin-pan; Actinin-α; Actinin α; α Actinin; α-Actinin; Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4; ACTN4; α-Actinin4; Actinin α4; Actininα4; αActinin-4; Actinin-α4; α-actinin-4; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; Non-muscle alpha-actinin-1; ACTN1; α-Actinin1; Actinin α1; Actininα1; αActinin-1; Actinin-α1; α-actinin-1; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; ACTN2; α-Actinin2; Actinin α2; Actininα2; αActinin-2; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; α-Actinin3; Actinin α3; Actininα3; αActinin-3; Actinin-α3; α-actinin-2; α-actinin-3
Immunogen: Synthetic peptide, corresponding to amino acids 30-60 of Human Actinin-α1.
Conjugate: Unconjugated
Modification: Unmodified