Actinin-α3 (E25) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS2205-50, BS2205-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q08043
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF
All Applications: WB: 1:500~1:1000
IF: 1:50~1:200
Background: The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Actinin-α3 (E25) polyclonal antibody detects endogenous levels of actinin-α3 protein, this antibody does not cross-react with other α-actinin isoforms.
Bioworld Molecular Weight: ~ 103 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Actinin-α3 (E25) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse muscle tissue lysate
Lane 3: Rat muscel tissue lysate, Immunohistochemistry (IHC) analyzes of Actinin-α3 (E25) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Actinin-α3 (E25) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50, showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; α-Actinin3; Actinin α3; Actininα3; αActinin-3; Actinin-α3; α-actinin-3
Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Actinin-α3.
Conjugate: Unconjugated
Modification: Unmodified