ACTN3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75014-50, BS75014-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q08043
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200
Background: This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 103kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ACTN3 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunohistochemistry of paraffin-embedded mouse skeletal muscle using ACTN3 Rabbit pAb at dilution of 1:500. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat skeletal muscle using ACTN3 Rabbit pAb at dilution of 1:500. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Alternative Name: ACTN3
Immunogen: Recombinant fusion protein of human ACTN3(NP_001095.2).
Conjugate: Unconjugated
Modification: Unmodified