ACTR3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS5620-50, BS5620-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P61158
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ACTR3 polyclonal antibody detects endogenous levels of ACTR3 protein.
Bioworld Molecular Weight: ~ 48 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ACTR3 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of ACTR3 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50, showing cytoplasmic and perinuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ACTR3 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50, showing cytoplasmic and perinuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Actin-like protein 3; ARP3; Actin-related protein 3
Immunogen: A synthetic peptide corresponding to residues in Human ACTR3.
Conjugate: Unconjugated
Modification: Unmodified