ADAM10 Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS71726-50, BS71726-100
Swiss-Prot: O14672
Host: Rabbit
ADAM10 Polyclonal Antibody-BS71726
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 90KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of RAW264.7, using ADAM10 antibody at 1:1740 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s., Immunofluorescence analysis of HeLa cells using ADAM10 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ADAM10; AD10; AD18; CD156c; CDw156; HsT18717; MADM; RAK; kuz
Immunogen: Recombinant fusion protein of human ADAM10(NP_001101.1).
Modification: Unmodified
Conjugate: Unconjugated