ADCY 5/6 (F1052) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS2208-50, BS2208-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O95622/O43306
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200
Background: A cyclase V, also known as ADCY5, is a 1,261 amino acid Adenylyl cyclase that localizes to cellular membranes and contains two guanylate cyclase domains. Similar to other A cyclase proteins, A cyclase V uses magnesium as a cofactor to catalyze the conversion of ATP to cAMP. A cyclase VI, also known as ADCY6 (adenylate cyclase type 6), is a 1,168 amino acid A cyclase that localizes to the membrane and contains two guanylate cyclase domains. Using magnesium as a cofactor, A cyclase VI functions as a calcium-inhibitable A cyclase that catalyzes the conversion of ATP to 3',5'-cyclic AMP and diphosphate and plays a role in a variety of events throughout the body. Multiple isoforms of A cyclase VI exist due to alternative splicing events.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ADCY 5/6 (F1052) polyclonal antibody detects endogenous levels of Adenylate cyclase type 5 and Adenylate cyclase type 6 protein.
Bioworld Molecular Weight: ~ 130, 139 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ADCY 5/6 (F1052) polyclonal antibody at 1:500 dilution
Lane 1: Panc1 whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: Hela whole cell lysate (40ug)
Lane 5: MCF-7 whole cell lysate (40ug)
Lane 6: The Pancreas tissue lysate of Mouse (40ug)
Lane 7: The Pancreas tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of ADCY 5/6 (F1052) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ADCY 5/6 (F1052) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: A cyclase V; A cyclase VI; Adenylate cyclase type 5; ATP pyrophosphate-lyase 5; Adenylate cyclase type V; Adenylyl cyclase 5; ADCY5; Adenylate cyclase type 6; ATP pyrophosphate-lyase 6; Adenylate cyclase type VI; Adenylyl cyclase 6; Ca(2+)-inhibitable adenylyl cyclase; ADCY6; KIAA0422
Immunogen: Synthetic peptide, corresponding to amino acids 1111-1160 of Human ADCY 5
Conjugate: Unconjugated
Modification: Unmodified