ADD2 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-92932-60, E-AB-92932-120, E-AB-92932-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: ADD2
Target Synonym: ADD2; ADDB; adducin 2
Research Areas: Cardiovascular, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P35612
Background: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human ADD2
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:1000-1:2000
Calculated MW: 11kDa/31kDa/62kDa/64kDa/66kDa/72kDa/80kDa
Observed MW: 81kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only