ADH1A/ADH1B/ADH1C Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS77827-50, BS77827-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P07327/P00325/P00326
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: Alcohol dehydrogenase,consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 40kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of Mouse lung, using ADH1A/ADH1B/ADH1C Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of Human liver using ADH1A/ADH1B/ADH1C Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of Rat liver using ADH1A/ADH1B/ADH1C Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of Mouse liver using ADH1A/ADH1B/ADH1C Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ADH1A/ADH1B/ADH1C
Immunogen: A synthetic peptide of human ADH1A/ADH1B/ADH1C(NP_000659.2).
Conjugate: Unconjugated
Modification: Unmodified