ADH7 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS5600-50, BS5600-100
Sizes: 50µl, 100µl
Swiss-Prot: P40394
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: ADH7 (alcohol dehydrogenase 7 (class IV), µ or σ polypeptide), is a 386 amino acid protein belonging to the zinc-containing alcohol dehydrogenase family and the class-IV subfamily. Seven different human ADH isozymes exist: three belong to class-I: α, β and γ; one to class-II: π; one to class-III: χ; one to class-IV: ADH7; and one to class-V: ADH6. Encoded by a gene that maps to human chromosome 4q23, ADH7 localizes to the cytoplasm and is preferentially expressed in stomach, but, unlike other family members, is absent from liver. ADH7 is a homodimer that binds two zinc ions per subunit and contains nine exons. ADH7 participates in the synthesis of retinoic acid, a hormone important for cellular differentiation. Variations in ADH7 may be associated with alcohol dependence. ADH7 may also play a role in protection against aerodigestive tract cancer.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ADH7 polyclonal antibody detects endogenous levels of ADH7 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ADH7 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of ADH7 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 40 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Alcohol dehydrogenase class 4 mu/sigma chain; Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Retinol dehydrogenase; ADH7
Immunogen: A synthetic peptide corresponding to residues in Human ADH7.
Conjugate: Unconjugated
Modification: Unmodified