ADRBK2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS6514-50, BS6514-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P35626
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200
Background: The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 100kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ADRBK2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s., Immunohistochemistry of paraffin-embedded rat spinal cord using ADRBK2 Antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using ADRBK2 Antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Alternative Name: GRK3; ADRBK2; BARK2
Immunogen: Recombinant fusion protein of human ADRBK2(NP_005151.2).
Conjugate: Unconjugated
Modification: Unmodified