AHNAK Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS78040-50, BS78040-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q09666
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC, IF/ICC
All Applications: IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200
Background: The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Immunogen: Recombinant fusion protein of human AHNAK(NP_001611.1).
Conjugate: Unconjugated
Modification: Unmodified
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry of paraffin-embedded Mouse liver using AHNAK antibody at dilution of 1:50.1.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Mouse brain using AHNAK antibody at dilution of 1:50.1.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of NIH/3T3 cells using AHNAK antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of A-549 cells using AHNAK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.