AhR (K32) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: AP0420-50, AP0420-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P35869
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AhR (K32) polyclonal antibody detects endogenous levels of AhR protein.
Bioworld Molecular Weight: ~ 94 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AhR (K32) polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of AhR (K32) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of AhR (K32) pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR; BHLHE76
Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human AhR.
Conjugate: Unconjugated
Modification: Unmodified