AIFM2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS67629-50, BS67629-100
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: Q9BRQ8
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IHC, IF/ICC
All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)
Background: Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of AIFM2 protein.
Bioworld Molecular Weight: ~ 43，55 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AIFM2 expression in mouse liver (A), Hela (B), A549 (C), HepG2 (D) whole cell lysates., Immunohistochemical analysis of AIFM2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of AIFM2 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Alternative Name: AMID; PRG3; Apoptosis-inducing factor 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; p53-responsive gene 3 protein
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AIFM2. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified