AKAP3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS76809-50, BS76809-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O75969
Host: Rabbit
Reactivity: Mouse, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of rat testis, using AKAP3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 115kDa
Alternative Name: AKAP3; AKAP 110; AKAP110; CT82; FSP95; HEL159; PRKA3; SOB1
Immunogen: Recombinant fusion protein of human AKAP3(NP_006413.3).
Conjugate: Unconjugated
Modification: Unmodified