AKAP5 (E7) Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS1962-50, BS1962-100
Sizes: 50µl, 100µl
Swiss-Prot: P24588
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regulatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell. Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regulatory subunit (RIα, RIIα, RIβ, or RIIβ), individual AKAPs contain additional domains responsible for the recrutiment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinase, etc.) or restricting AKAP to a specific subcellular location. AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities. AKAP5 specifically binds to the regulatory subunit of PKAIIβ, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions. The other binding domains of AKAP5 have been shown to interact with calmodulin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca2+-sensing pathways in various cell types.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: AKAP5 (E7) polyclonal antibody detects endogenous levels of AKAP5 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AKAP5 (E7) pAb at 1:500 dilution
Lane 1: MEF whole cell lysate (40ug)
Lane 2: C6 whole cell lysate (40ug)
Lane 3: Hela whole cell lysate (40ug)
Lane 4: A549 whole cell lysate (40ug)
Lane 5: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of AKAP5 (E7) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Bioworld Molecular Weight: ~ 47, 79 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: A kinase (PRKA) anchor protein 5; A kinase anchor protein 5; A kinase anchor protein 79kDa; A-kinase anchor protein 5; A-kinase anchor protein 79 kDa; AKAP 5; AKAP 79; AKAP-5; AKAP5; AKAP75; AKAP79;
Immunogen: Synthetic peptide, corresponding to the N-terminal of Human AKAP 5.
Conjugate: Unconjugated
Modification: Unmodified