AKR1B1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB0032-50, MB0032-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P15121
Host: Mouse
Reactivity: Human
Applications: WB ICC
All Applications: WB: 1:1000
ICC: 1:50~200
Background: Aldose reductase (also designated AKR1B1, ALDR1, ALR2 or AR) is member of the monomeric NADPH-dependent aldoketoreductase family. Aldose reductase, which has a molecular mass of 36 kDa, catalyzes the reduction of various aldehydes and has been implicated in the development of diabetic complications by catalyzing the reduction of the aldehyde form of glucose, to the corresponding sugar alcohol, sorbitol. This pathway plays a minor role in glucose metabolism in most tissues, however in diabetic hyperglycemia, cells undergoing insulin-independent uptake of glucose accumulate significant quantities of sorbitol. The resulting hyperosmotic stress to cells may be a cause of diabetic complications such as neuropathy, retinopathy, and cataracts. Aldose reductase is very similar to human aldehyde reductase, bovine prostaglandin F synthase and to the European common frog protein, rho-crystallin.
Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of AKR1B1 and does not cross-react with related proteins.
Bioworld Molecular Weight: Predicted band size:36KDa
Observed band size:36KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western BlotDetection of AKR1B1 in HT1080, MCF7, A431&Hela cell lysates using AKR1B1 antibody (1:1000 diluted).
Alternative Name: Aldose reductase; ldehyde reductase; Aldo-keto reductase family 1 member B1; AKR1B1; ALDR1
Immunogen: Recombinant full length Human AKR1B1.
Conjugate: Unconjugated
Modification: Unmodified